Arachis hypogaea Lectin (PNA) - AP (Alkaline Phosphatase)

Product Description

Arachis hypogaea agglutinin (PNA) is purified by affinity chromatography (SKU: 21510000) and is consisting of four subunits of approximately 27,000 each. This lectin has an isoelectric point between pH 5.5 and pH 6.5. It has a carbohydrate specificity for Galβ3GalNAc with an eluting sugar of galactose. Galactose and lactose are poor inhibitors while disaccharide Gal β(1->3)GalNAc and desialylated human erythrocyte N-substance (T-antigen which is present in many M and N blood groups) are potent inhibitors of PNA. Alkaline phosphatase (AP) is conjugated to Arachis hypogaea (Peanut) Lectin (PNA) to show the binding of PNA in many applications including Western blotting and ELISA. Alkaline phosphatase is a large protein (140 kDa) that catalyzes the hydrolysis of phosphate groups from a substrate resulting in a colored or fluorescent product. The optimal enzymatic activity of this protein is between pH 8 and 10, and its reaction rate remains linear, improving sensitivity over time.

Specifications:

• Source: Arachis hypogaea (Peanut)
• Activity: Less than 1 mg/ml will agglutinate human erythrocytes neuraminidase treatment of the cells.
• Protein Concentration (Based on OD 280): 1 mg purified PNA - AP / 1 mL buffer
• Carbohydrate Specificity: Galβ3GalNAc
• Inhibitory Carbohydrate: Galactose
• Divalent Ions Required: Ca²⁺, Mn²⁺
• Conjugation: Alkaline Phosphatase

Storage and Stability:

Store frozen at -20°C in amber vials or covered with foil in appropriate aliquot sizes. Avoid freeze thaw cycles. Can be stored at 2-8 °C for short term use. Clarify by centrifugation, if needed.