Arachis hypogaea Lectin (PNA) - FITC (Fluorescein)

Product Description

Arachis hypogaea agglutinin (PNA) is purified by affinity chromatography (SKU: 21510000) and is consisting of four subunits of approximately 27,000 each. This lectin has an isoelectric point between pH 5.5 and pH 6.5. It has a carbohydrate specificity for Galβ3GalNAc with an eluting sugar of galactose. Galactose and lactose are poor inhibitors while disaccharide Gal β(1->3)GalNAc and desialylated human erythrocyte N-substance (T-antigen which is present in many M and N blood groups) are potent inhibitors of PNA. Although extracts of Arachis hypogaea do not agglutinate untreated or trypsin-treated human erythrocytes, PNA has been known to agglutinate neuraminidase-treated human red blood cells. Arachis hypogaea (Peanut) Lectin (PNA) is labeled with fluorescein isothiocyanate (FITC) and has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin.

Specifications:

• Source: Arachis hypogaea (Peanut)
• Activity: Less than 1 mg/ml will agglutinate human erythrocytes neuraminidase treatment of the cells.
• Protein Concentration (Based on OD 280): 2 mg purified PNA - FITC / 1 mL buffer
• Carbohydrate Specificity: Galβ3GalNAc
• Inhibitory Carbohydrate: Galactose
• Divalent ions required: Ca²⁺, Mn²⁺
• Conjugation: FITC
• Fluorescence: Green
• Excitation: Maximum at 495-500 nm
• Emission: Maximum at 514-521 nm

Storage and Stability:

Store frozen at -20°C in amber vials or covered with foil in appropriate aliquot sizes. Avoid freeze thaw cycles. Can be stored at 2-8 °C for short term use. Clarify by centrifugation, if needed.