Arachis hypogaea Lectin (PNA) - Texas Red

Product Description

Arachis hypogaea agglutinin (PNA) is purified by affinity chromatography (SKU: 21510000) and is consisting of four subunits of approximately 27,000 each. This lectin has an isoelectric point between pH 5.5 and pH 6.5. It has a carbohydrate specificity for Galβ3GalNAc with an eluting sugar of galactose. Galactose and lactose are poor inhibitors while disaccharide Gal β(1->3)GalNAc and desialylated human erythrocyte N-substance (T-antigen which is present in many M and N blood groups) are potent inhibitors of PNA. Texas Red is a red-fluorescent dye and when bound to Arachis hypogaea Lectin (PNA) can show the binding pattern of this lectin in cellular imaging applications. There is very little overlap between the emission spectra of Texas Red and FITC making this combination ideal for dual-labeling experiments. Rhodamine dyes, such as Texas Red, are more photostable and less sensitive to pH change when compared to other dyes such as fluorescein. This product comes in a stabilized liquid form.

Specifications:

• Source: (Peanut)
• Activity: Less than 1 μg/ml will agglutinate human erythrocytes after neuraminidase treatment of the cells.
• Protein Concentration (Based on OD 280): 2 mg purified PNA - Biotin / 1 mL buffer
• Carbohydrate Specificity: Galactose
• Inhibitory Carbohydrate: Lactose
• Divalent Ions Required: Ca⁺⁺, Mg⁺⁺
• Conjugation: Texas Red
• Excitation: Maximum at 594
• Emission: Maximum at 615

Storage and Stability:

Store frozen at -20°C in amber vials or covered with foil in appropriate aliquot sizes. Avoid freeze thaw cycles. Can be stored at 2-8 °C for short term use. Clarify by centrifugation, if needed