Arachis hypogaea Lectin (PNA) - Biotinylated

Product Description

Arachis hypogaea agglutinin (PNA) is purified by affinity chromatography (SKU: 21510000) and is consisting of four subunits of approximately 27,000 each. This lectin has an isoelectric point between pH 5.5 and pH 6.5. It has a carbohydrate specificity for Galβ3GalNAc with an eluting sugar of galactose. Galactose and lactose are poor inhibitors while disaccharide Gal β(1->3)GalNAc and desialylated human erythrocyte N-substance (T-antigen which is present in many M and N blood groups) are potent inhibitors of PNA. Biotin is a small molecule involved in a wide range of metabolic processes. This ligand forms a complex with Avidin and Streptavidin, resulting in the strongest non-covalent protein-ligand interaction known. Biotinylated Arachis hypogaea (Peanut) Lectin (PNA) has an appropriate amount of biotin bound to provide optimum detection characteristics when using an Avidin-HRP or Streptavidin-HRP conjugate. Biotinylated lectins allow for more sensitive detection in ELISA and Western-blotting applications.

Specifications:

• Source: (Peanut)
• Activity: Less than 1 μg/ml will agglutinate human erythrocytes after neuraminidase treatment of the cells.
• Protein Concentration (Based on OD 280): 5 mg purified PNA - Biotin / 1 mL buffer
• Carbohydrate Specificity: Galactose
• Inhibitory Carbohydrate: Lactose
• Divalent Ions Required: Ca⁺⁺, Mg⁺⁺
• Conjugation: Biotin

Storage and Stability:

Store frozen at -20°C in amber vials or covered with foil in appropriate aliquot sizes. Avoid freeze thaw cycles. Can be stored at 2-8 °C for short term use. Clarify by centrifugation, if needed.