Arachis hypogaea Lectin (PNA) - Cy5

Product Description

Arachis hypogaea agglutinin (PNA) is purified by affinity chromatography (SKU: 21510000) and is consisting of four subunits of approximately 27,000 each. This lectin has an isoelectric point between pH 5.5 and pH 6.5. It has a carbohydrate specificity for Galβ3GalNAc with an eluting sugar of galactose. Galactose and lactose are poor inhibitors while disaccharide Gal β(1->3)GalNAc and desialylated human erythrocyte N-substance (T-antigen which is present in many M and N blood groups) are potent inhibitors of PNA. PNA may be used for clinical determination of T-polyagglutinability of erythrocytes and probing cell membranes of normal and malignant cells. This lectin can be of use in characterization of tumor-specific antigens on the surface of malignant cells. It can be employed in the fractionation of stem cells in mice for use in bone marrow transplantation across histocompatibility barriers. Cy5, when bound to Arachis hypogaea Lectin (PNA), can show the binding pattern of this lectin in cellular imaging and flow cytometry. The excitation wavelength required for Cy5 to fluoresce is high enough to avoid overlap with most other fluorochromes, making it useful for dual-labeling experiments. Because of this high excitation, there is typically less background from autofluorescence of biological specimens. This product comes in a stabilized liquid form.

Specifications:
• Source: Arachis hypogaea (Peanut)
• Activity: Less than 1 µg/ml will agglutinate human erythrocytes neuraminidase treatment of the cells.
• Carbohydrate Specificity: Terminal ß-Galactose.
• Inhibitory Carbohydrate: Lactose > ß-Galactose.
• Mitogenic Activity: No
• Divalent Ions Required: Ca2+, Mg2+
• Conjugation: Cy5
• Excitation: 650 nm
• Emission: 670 nm

Storage and Stability:
Store frozen at -20°C in amber vials or covered with foil in appropriate aliquot sizes. Avoid freeze thaw cycles. Can be stored at 2-8°C for short term use.