Artocarpus integrifolia Lectin (AIA) - FITC (Fluorescein)

Product Description

Artocarpus integrifolia Lectin (AIA) is purified by affinity chromatography (SKU: 21510001) and is tetrameric two-chain structure consisting of four identical protomers with an isoelectric point of pH 7.8. The lectin has specificity for blood group types O (+SA) and T. Jacalin is considered a galactose-specific lectin and elutes with galactose or melibiose. A post-translational proteolytic modification of Jacalin gives the lectin a novel carbohydrate-binding site involving the N terminus of the α-chain. The protein's structure explains its carbohydrate-binding specificity of T-antigen disaccharide Galß1,3GalNAc. Potent inhibitors of jacalin include D-galactose, ß-Met-Gal and 2-deoxy-α-D-galactose. Artocarpus integrifolia Lectin (AIA) is labeled with fluorescein isothiocyanate (FITC) and has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. FITC conjugates have been used in a variety of immunohistochemical and flow cytometry applications. This product comes in a stabilized liquid form.

Specifications:

• Source: Artocarpus integrifolia (Jackfruit)
• Activity: Less than 0.5 ug /ml will agglutinate neuraminidase treated human erythrocytes.
• Protein Concentration (Based on OD 280): 1 mg purified AIA FITC / 1 mL buffer
• Carbohydrate Specificity: Galß1-3GalNAc
• Inhibitory Carbohydrate: Galactose or Melibiose
• Divalent ions required: None
• Mitogenic Activity: Yes
• Conjugation: FITC
• Excitation: Maximum at 495-550 nm
• Emission: Maximum at 514-521 nm

Storage and Stability:

Store frozen at -20°C in amber vials or covered with foil in appropriate aliquot sizes. Avoid freeze thaw cycles. Can be stored at 2-8°C for short term use.