Lens culinaris Lectin (LCA/LCH) - Semi-Pure

Lens culinaris Lectin (LCA/LCH) - Semi-Pure

Price: Regular price $125.70

SKU#: 21511390-1

Product Description

Fractionated Lens culinaris lectin (LCA) is composed of four subunits that consists of an ?-chain (MW: 5.7 kDa) and ?-chain (MW: 17.5 kDa). It has a carbohydrate specificity toward ?-D-mannose and ?-D-glucose, eluting with the sugar Me?Man+Me?Glc. This lectin is useful in affinity chromatography columns for the separation of glycoconjugates. The two isomers LCA-A and LCA-B agglutinates human red blood cells, although not blood group specific. Activity of LCA is determined by haemagglutination with human blood and agglutinates a 2% suspension of human erythrocytes at a lectin concentration of 8µg/ml. LCA requires calcium and manganese ions for binding. See other LCA conjugates.

Technical Specifications

Abbreviation: LCA/LCH
Material Source:: Lentil
Conjugate:: None
Appearance Form:: Powder
Preferred Sugar Specificity:: α-Mannose, α-Glucose
Inhibiting or Eluting Sugar:: α-Methylmannoside + α-Methylglucoside
Divalent Ions:: Ca++, Mn++
Mitogenic Activity:: Yes
Storage Temperature:: -20°C
Hazardous Shipping:: Non-hazardous

Applications

References

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