Rapid Western Blotting Kit Mouse-Semi Dry Transfer

Product Description

Enables Western Blotting preparation and development in less than 90 minutes. The Rapid Western Blotting Kit provides reagents for all blotting steps from gel transfer, blocking and a combined 1º and 2º antibody incubation step. Users provide the primary antibody and chemiluminescent substrates.

One convenient kit includes all of the following specialized reagents:

Rapid Transfer Buffer, 10X

Transfer from gel to PVDF or nitrocellulose membrane in 10-20 minutes, Use with wet or semi-dry transfer apparatus, Methanol-free, non-hazardous formulation

RapidBlock™ Solution, 10X

Blocks PVDF and nitrocellulose membranes in just 5 minutes, Protein-free formulation reduces cross-reactivity, Enhances chemiluminescent signal

Rapid Antibody Diluent, 10X

One-step combined primary and secondary antibody incubation reduces total incubation time to 30-45 minutes, Secondary HRP Conjugated Antibodies, Choose from either anti-mouse or anti-rabbit antibodies

Rapid Wash Solution, 20X

Reduces wash time to a total of 15 minutes

Kits are supplied for either standard wet or semi-dry transfer apparatus and with either anti-mouse or anti-rabbit secondary antibodies.