Artocarpus integrifolia Lectin (AIA) - Semi-Pure

Product Description

Artocarpus integrifolia Lectin (AIA) is purified by fractionation and is tetrameric two-chain structure consisting of four identical protomers with an isoelectric point of pH 7.8. The lectin has specificity for blood group types O (+SA) and T. Jacalin is considered a galactose-specific lectin and elutes with galactose or melibiose. A post-translational proteolytic modification of Jacalin gives the lectin a novel carbohydrate-binding site involving the N terminus of the α-chain. The carbohydrate-binding specificity of AIA to the Thomsen-Friedenreich antigen (TF-Galß1-3GalNAc) gives it antitumor potential, as this antigen is expressed in more than 85% of human carcinomas. Jacalin has shown antiproliferative effects on colonal epithelial cells when administered to carcinogen-exposed mice, suggesting potential in colorectal cancer. Potential pathways of anticancer effect include COX-2 inhibition by Jacalin and apoptotic/necrotic cell death induced by high levels of TNF-α from Jacalin-induced macrophages. Jacalin has also been suggested as a potential avenue of study in novel SARS-CoV2 research, as it can recognize the receptor-binding domain (RBD) of the virus and induce a conformational change. Potent inhibitors of jacalin include D-galactose, ß-Met-Gal and 2-deoxy-α-D-galactose. This product comes in a stable lyophilized form.

Specifications:

• Source: Artocarpus integrifolia (Jackfruit)
• Activity: Less than 0.5 ug /ml will agglutinate neuraminidase treated human erythrocytes.
• Carbohydrate Specificity: Galß1-3GalNAc
• Inhibitory Carbohydrate: Galactose or Melibiose

• Mitogenic Activity: Yes

• Divalent ions required: None

Storage and Stability:

Store frozen at -20°C in amber vials or covered with foil in appropriate aliquot sizes. Avoid freeze thaw cycles. Can be stored at 2-8°C for short term use.