Rapid Western Blotting Kit Rat-Wet Transfer

Product Description

Synonym(s): Western blotting kit

The Rapid Western Blotting Kit provides reagents for all blotting steps from gel transfer, blocking and a combined 1° and 2° antibody incubation step. Users provide the primary antibody and chemiluminescent substrates.

Features:

Enables Western Blotting preparation and development in less than 90 minutes.

One convenient kit includes all of the following specialized reagents:

Rapid Transfer Buffer, 10X

Transfer from gel to PVDF or nitrocellulose membrane in 10-20 minutes

Use with wet or semi-dry transfer apparatus

Methanol-free, non-hazardous formulation

RapidBlock™ Solution, 10X

Blocks PVDF and nitrocellulose membranes in just 5 minutes

Protein-free formulation reduces cross-reactivity

Enhances chemiluminescent signal

Rapid Antibody Diluent, 10X

One-step combined primary and secondary antibody incubation reduces total incubation time to 30-45 minutes

Secondary HRP Conjugated Antibodies

Choose from either anti-mouse or anti-rabbit antibodies

Rapid Wash Solution, 20X

Reduces wash time to a total of 15 minutes

Kits are supplied for either standard wet or semi-dry transfer apparatus and with either anti-mouse or anti-rabbit secondary antibodies.