Artocarpus integrifolia Lectin (AIA) - Biotinylated

Product Description

Artocarpus integrifolia Lectin (AIA) is purified by affinity chromatography (SKU: 21510001) and is tetrameric two-chain structure consisting of four identical protomers with an isoelectric point of pH 7.8. The lectin has specificity for blood group types O (+SA) and T. Jacalin is considered a galactose-specific lectin and elutes with galactose or melibiose. A post-translational proteolytic modification of Jacalin gives the lectin a novel carbohydrate-binding site involving the N terminus of the α-chain. The protein's structure explains its carbohydrate-binding specificity of T-antigen disaccharide Galß1,3GalNAc. Potent inhibitors of jacalin include D-galactose, ß-Met-Gal and 2-deoxy-α-D-galactose. Biotin is a small molecule involved in a wide range of metabolic processes. This ligand forms a complex with Avidin and Streptavidin, resulting in the strongest non-covalent protein-ligand interaction known. Biotinylated AIA has an appropriate amount of biotin bound to provide optimum detection characteristics when using an Avidin-HRP or Streptavidin-HRP conjugate. Biotinylated lectins allow for more sensitive detection in ELISA and Western-blotting applications

Specifications:

• Source: Artocarpus integrifolia (Jackfruit)
• Activity: Less than 0.5 ug /ml will agglutinate neuraminidase treated human erythrocytes.
• Protein Concentration (Based on OD 280): 2 mg purified AIA- Biotin / 1 mL buffer
• Carbohydrate Specificity: Galß1-3GalNAc
• Inhibitory Carbohydrate: Galactose or Melibiose
• Mitogenic Activity: Yes
• Divalent ions required: None
• Conjugation: Biotin

Storage and Stability:

Store frozen at -20°C in amber vials or covered with foil in appropriate aliquot sizes. Avoid freeze thaw cycles. Can be stored at 2-8°C for short term use.